Thermally programmed one-pot CRISPR assay for on-site pandemic surveillance
8.6
来源：
Nature
关键字：
point-of-care diagnostics
发布时间：
2025-11-21 23:42
摘要：
TRACE is a novel CRISPR-based diagnostic assay designed for rapid and accurate detection of monkeypox virus (MPXV). It operates in a one-pot format, significantly reducing complexity and time to results. The assay demonstrates a limit of detection of 2.5 copies/test and achieves 99.5% accuracy across various clinical samples. Its design allows for simultaneous detection of pathogen and host genes, addressing critical limitations of existing CRISPR assays. TRACE meets ASSURED criteria, making it suitable for deployment in resource-limited settings, thus enhancing pandemic preparedness.
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domain_focus
1.0分+
business_impact
1.0分+
scientific_rigor
1.5分+
timeliness_innovation
1.5分+
investment_perspective
2.5分+
market_value_relevance
1.0分+
team_institution_background
0.5分+
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1.0分+
关键证据
TRACE achieves 99.5% accuracy across diverse sample types.
The assay can detect MPXV within 11 minutes, significantly faster than conventional methods.
TRACE meets ASSURED criteria for point-of-care testing.
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AI评分总结
TRACE is a novel CRISPR-based diagnostic assay designed for rapid and accurate detection of monkeypox virus (MPXV). It operates in a one-pot format, significantly reducing complexity and time to results. The assay demonstrates a limit of detection of 2.5 copies/test and achieves 99.5% accuracy across various clinical samples. Its design allows for simultaneous detection of pathogen and host genes, addressing critical limitations of existing CRISPR assays. TRACE meets ASSURED criteria, making it suitable for deployment in resource-limited settings, thus enhancing pandemic preparedness.