RoCK and ROI: single-cell transcriptomics with multiplexed enrichment of selected transcripts and region-specific sequencing
8.0
来源：
Nature
关键字：
CRISPR
发布时间：
2025-12-10 23:41
摘要：
RoCK and ROI are innovative single-cell RNA sequencing methods that enhance the detection of specific transcripts and splice junctions. The study validates these methods across various biological systems, including murine and human cells, demonstrating their potential in cancer research. By combining targeted capture and sequencing, RoCK and ROI significantly improve transcript detection rates, achieving robust results in up to 98% of cells. This advancement positions RoCK and ROI as a versatile tool for exploring complex biological mechanisms in health and disease.
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domain_focus
1.0分+1.0分+符合生物技术领域
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0.8分+展示了潜在的商业应用
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1.5分+有具体实验数据支持
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1.0分+与高发疾病相关
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1.0分+技术壁垒高
关键证据
RoCK and ROI enables retrieval of specific sequence information without compromising overall single cell transcriptome information.
The method can achieve robust target detection in up to 98% of cells.
RoCK and ROI is the only method combining targeted capture directly at the RNA level with specific detection of features of interest.
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AI评分总结
RoCK and ROI are innovative single-cell RNA sequencing methods that enhance the detection of specific transcripts and splice junctions. The study validates these methods across various biological systems, including murine and human cells, demonstrating their potential in cancer research. By combining targeted capture and sequencing, RoCK and ROI significantly improve transcript detection rates, achieving robust results in up to 98% of cells. This advancement positions RoCK and ROI as a versatile tool for exploring complex biological mechanisms in health and disease.