iCAT as an open-source platform for axial rotation and high-resolution imaging of organoids and living organisms
iCAT is an open-source multifunctional accessory designed to enhance light microscopy by enabling axial rotation and precise temperature control for imaging organoids and live specimens. Its modular design allows for easy 3D printing and assembly, making it accessible to researchers. The device significantly improves the capabilities of traditional microscopy, facilitating detailed studies of dynamic cellular processes and complex developmental phenomena, particularly in model organisms like zebrafish.
Elucidation of puberulic acid–induced nephrotoxicity using stem cell-based kidney organoids
本研究开发了一种基于干细胞的肾脏类器官模型，用于评估puberulic酸的肾毒性。研究发现puberulic酸在小鼠模型中诱导急性肾损伤，表现为血清肌酐升高和组织学变化。该模型能够重现已知肾毒素的损伤特征，为早期药物测试提供了潜在的替代方案。
EZH2 expression in hepatocellular carcinoma and its relationship with circadian rhythm-related genes
The study investigates the role of EZH2 in hepatocellular carcinoma (HCC), revealing its overexpression correlates with advanced tumor stages and poor patient prognosis. It establishes a link between EZH2 and circadian rhythm-related genes, suggesting that EZH2 may influence tumor progression through these pathways. The research utilizes data from TCGA and GEO databases, highlighting the potential of EZH2 as a prognostic biomarker and therapeutic target in HCC, with implications for personalized medicine.
Single-cell and bulk transcriptomics reveal Fusobacterium nucleatum promotes colorectal cancer metastasis by upregulating LAMC2 in in vitro and in vivo models
本研究揭示了Fusobacterium nucleatum通过上调LAMC2促进结直肠癌（CRC）转移的机制，表明LAMC2可能成为潜在的治疗靶点。研究显示，LAMC2在CRC组织中的高表达与患者预后不良显著相关，强调了肠道微生物在癌症进展中的重要作用。
Transcription factor CEBPA suppresses sepsis induced lung injury by activating SIRT4 transcription
本研究探讨了转录因子CEBPA和SIRT4在脓毒症诱导的急性肺损伤中的作用。结果显示，CEBPA通过激活SIRT4的转录，显著减轻了LPS诱导的细胞损伤，促进了细胞存活，减少了凋亡和炎症。这一发现为急性肺损伤的治疗提供了新的生物学靶点，具有重要的临床应用潜力。
A biomimetic senotherapy replenishing MAT2A promotes wound regeneration in preclinical models
该研究揭示了甲硫氨酸腺苷转移酶2A（MAT2A）在糖尿病伤口愈合中的关键作用，强调其在细胞衰老和慢性炎症中的影响。研究开发了一种基于生物仿生策略的纳米颗粒，用于靶向递送MAT2A自扩增RNA，显著促进伤口再生。该发现为糖尿病伤口治疗提供了新的潜在靶点，具有重要的临床应用前景。
The H3K27me3 reader UAD-2 recruits a TAF-12-containing transcription machinery to initiate piRNA expression within heterochromatic clusters
本研究探讨了UAD-2作为H3K27me3阅读器如何招募TAF-12及其他转录因子，促进C. elegans中piRNA的转录。研究表明，UAD-2与TAF-12的相互作用是piRNA转录复合物形成的关键，揭示了小RNA转录机制中的重要分子联系。
Microbial influx during early postnatal life fortifies the ocular surface and guards against allergic eye disease in mice
本研究探讨了小鼠眼结膜微生物群在早期发育过程中的动态变化及其对免疫系统的影响。结果表明，早期微生物群落的建立对眼表的发育至关重要，缺乏适当的微生物暴露可能导致过敏性眼病的易感性增加。这一发现为眼部过敏性疾病的预防和治疗提供了新的思路，强调了在新生儿使用抗生素时需谨慎考虑其对微生物群的潜在影响。
Restless legs syndrome and periodic limb movements of sleep — the relationship with stroke and other cerebrovascular disease
该研究探讨了不安腿综合症（RLS）与中风及其他脑血管疾病的关系，强调了其在脑血管风险评估中的潜在临床意义。研究指出RLS与中风之间存在双向关系，并揭示了多种共同的病理机制，包括神经递质失调、炎症、氧化应激等。这些发现为RLS的临床管理提供了新的视角，可能影响未来的治疗策略。
Exploring tumor dynamics and responses of prostate cancer to IL-27 based treatment combinations through biodynamic imaging and RNA sequencing analyses
该研究探讨了IL-27与化疗药物（如卡博替尼和多西他赛）联合治疗前列腺癌的效果，采用生物动态成像和RNA测序技术，揭示了肿瘤微环境的动态变化。研究表明，IL-27能够调节肿瘤微环境，增强免疫反应，从而提高治疗效果。这一创新方法为前列腺癌的治疗提供了新的思路，具有重要的临床和商业价值。
Serotonin receptor type 7 (5-HT7) promotes colorectal cancer progression by activating Wnt/β-catenin signaling via interacting with CK1ε
研究发现5-HT7受体在结直肠癌中显著上调，促进肿瘤细胞增殖、迁移和侵袭。通过与CK1ε相互作用，5-HT7激活Wnt/β-catenin信号通路，推动肿瘤进展。该发现为靶向5-HT7提供了新的治疗潜力，具有重要的临床应用价值。
Beyond swimming: emerging parameters for predicting the fertility of mouse spermatozoa
The review addresses the emerging parameters for predicting the fertility of mouse spermatozoa, particularly in the context of cryopreservation. It emphasizes the need for effective quality control methods to assess sperm functionality, which is crucial for maintaining genetically modified mouse lines in research. The article discusses various advanced techniques, including flow cytometry and computer-assisted sperm analysis, that could enhance the predictability of fertility outcomes. The authors call for standardized protocols to improve the reliability of sperm quality assessments in laboratory settings.
A systematic review of explainable artificial intelligence methods for speech-based cognitive decline detection
该系统评估了可解释人工智能（XAI）在基于语音的认知衰退检测中的应用，强调了透明性和可解释性在临床环境中的重要性。研究回顾了13项相关研究，探讨了不同XAI技术的有效性和临床适用性，发现这些技术能够有效识别阿尔茨海默病和轻度认知障碍的早期迹象。尽管取得了一定的进展，但在临床验证和真实世界应用方面仍存在显著差距。
An autoencoder and vision transformer based interpretability analysis on the performance differences in automated staging of second and third molars
本研究探讨了第二和第三磨牙的自动分期性能差异，提出了一种结合自编码器和视觉变换器的框架，旨在提高牙齿分期的准确性和透明度。研究表明，该框架在牙齿37和38的分期准确性上均有显著提升，尤其是在处理高内部变异性数据时。通过分析潜在空间，研究揭示了牙齿38数据集的高内部变异性和低外部可分性，强调了数据中心问题对模型性能的影响。该框架的透明性和诊断能力为法医应用提供了重要支持。
In silico design of novel multi-epitope peptide vaccine against Neospora caninum induced cattle abortion targeting extracellular GRA2 and Nc-p43 protein
本研究设计了一种针对Neospora caninum的多表位肽疫苗，采用生物信息学方法构建，目标为关键的致病蛋白GRA2和Nc-p43。研究表明，该疫苗具有良好的免疫原性和稳定性，能够激发强烈的免疫反应，适合早期投资。该疫苗的开发可能为控制牛只流产和相关经济损失提供有效的解决方案。