Programmable no-nonspecific genetic analytical system via dual-circle-based rolling circle amplification with an efficient CRISPR/Cas12a biosensing strategy
研究开发了一种新型的CRISPR/Cas12a系统，结合无非特异性扩增的双环滚环扩增策略，显著提高了对p53基因的检测灵敏度，达到0.77 fM。该方法在临床样本中表现出良好的应用潜力，与传统qPCR方法结果一致，显示出在癌症早期筛查和诊断中的重要性。
NEDD4L-mediated Gasdermin D and E ubiquitination regulates cell death and tissue injury
NEDD4L作为一种E3泛素连接酶，在调节Gasdermin D和E的稳定性及其功能方面发挥关键作用。研究发现，NEDD4L缺失会导致细胞死亡和组织损伤，尤其是在肾脏和肺部，可能与多种疾病的发生发展相关。这一发现为生物技术领域提供了新的研究方向，尤其是在开发针对相关疾病的治疗策略方面。
Reconstruction of the lymphatic system by transplantation of a centrifuge-based bioengineered lymphatic tissue
本研究展示了一种新型的离心细胞堆叠技术，成功构建了生物工程淋巴组织（CeLyTs），并在淋巴结切除后的小鼠模型中证明了其对继发性淋巴水肿的长期治疗效果。CeLyTs通过再生淋巴结结构，显著改善了淋巴流动，可能为淋巴水肿的治疗提供新的解决方案。
A novel peptide-based strategy to enhance GBA1 expression for treating Parkinson’s disease
Hirunipin 4, a peptide derived from leeches, has been identified as a novel therapeutic candidate for Parkinson's disease. This study demonstrates that hirunipin 4 enhances GBA1 expression, increases glucocerebrosidase (GCase) activity, and reduces pathological α-synuclein aggregation in neuronal models. Mechanistically, it promotes nuclear translocation of TFEB, a key regulator of lysosomal function, and stabilizes GCase protein levels. The findings suggest that hirunipin 4 could serve as a versatile treatment option for addressing lysosomal dysfunction in neurodegenerative diseases.
Immunohistochemical analysis of filamin a expression in acromegaly and its correlation with tumor characteristics and treatment response
本研究通过免疫组化分析探讨了FLNA在生长激素分泌的垂体肿瘤中的表达及其与肿瘤特征和治疗反应的相关性。结果显示，FLNA在所有34个样本中均呈阳性，且其表达强度与肿瘤的侵袭性特征相关。研究还发现FLNA与SSTR5的表达显著相关，提示其可能在治疗反应中发挥作用。尽管样本量较小，但这些发现为未来的研究提供了基础，尤其是在个性化治疗策略的开发方面。
Mesenchymal-to-epithelial transition of perivascular cells contributes to endometrial re-epithelialization
研究发现Nestin+血管周围细胞在子宫内膜再上皮化中发挥重要作用，揭示了雌激素和Notch信号通路在该过程中的调控机制。这一发现为女性生殖健康提供了新的治疗思路，尤其在处理子宫相关疾病方面，具有重要的临床应用潜力。
Smurf1 promotes gastric cancer progression by regulating Axin2-dependent Wnt signaling pathway
研究表明，SMAD特异性E3泛素蛋白酶1（Smurf1）在胃癌中作为一种致癌基因，通过调节Axin2的泛素化和降解，促进Wnt/β-catenin信号通路的激活，从而加速胃癌的进展。Smurf1的高表达与患者的无病生存期显著相关，提示其可能成为胃癌治疗的新靶点。
Mitochondria relay cholesterol signal exacerbates osteoarthritis in mice
本研究探讨了胆固醇代谢与骨关节炎之间的关系，发现胆固醇通过激活线粒体转移信号加剧骨关节炎的进展。研究表明，线粒体在关节炎中的作用不仅限于代谢调节，还涉及炎症信号的传递。靶向线粒体Nudt8的药物（如pantethine）显示出改善骨关节炎症状的潜力，提示其作为新型治疗策略的可能性。
Targeting dual-specificity phosphatase 23 to overcome chemoresistance and stem cell-like behavior in non-small cell lung cancer cells
研究发现，双特异性磷酸酶23（DUSP23）在非小细胞肺癌（NSCLC）中发挥关键作用，特别是在化疗耐药性和癌症干细胞样特性方面。DUSP23的表达与患者预后显著相关，尤其在接受化疗的患者中。通过靶向DUSP23，有望克服化疗耐药性并改善患者的生存率，显示出其作为潜在治疗靶点的价值。
Tumor cell-intrinsic PD-1 regulates chemotherapy resistance in colorectal cancer cells by activating downstream MAPK signaling
The research investigates the role of tumor cell-intrinsic PD-1 (ciPD-1) in colorectal cancer (CRC), revealing that high ciPD-1 levels correlate with chemotherapy resistance and poor patient outcomes. The study demonstrates that ciPD-1 activates MAPK signaling pathways, enhancing cell survival under chemotherapy stress. Inhibiting ciPD-1 increases sensitivity to chemotherapy, suggesting that targeting ciPD-1 could improve treatment efficacy in CRC patients. This highlights the potential for innovative therapeutic strategies combining PD-1 inhibitors with conventional chemotherapy.
Circular single stranded DNA potentiates non-viral gene insertion in hematopoietic stem and progenitor cells
研究表明，圆形单链DNA（CssDNA）作为非病毒DNA供体模板在造血干细胞（HSPCs）中的基因插入效率显著提高，达到40%以上。与传统的腺病毒载体（AAV）相比，CssDNA编辑的HSPCs在小鼠模型中表现出更高的移植能力和基因插入事件的维持。这一发现为基因治疗提供了新的方向，特别是在HSPCs和T细胞工程中，展示了CssDNA在未来细胞和基因治疗中的应用潜力。
Acetyl-CoA carboxylase 1 knockdown promotes esophageal squamous cell carcinoma metastasis via the CXCL8–NET axis
研究发现，ACC1的下调通过增强CXCL8的表达，促进食管鳞状细胞癌（ESCC）的迁移和侵袭。ACC1的低表达与患者的预后不良相关，提示其作为潜在的治疗靶点和预后标志物。该研究强调了ACC1-CXCL8轴在ESCC转移中的关键作用，可能为未来的治疗策略提供新的方向。
Exploring biomarkers related to coagulation and immunity in myocardial infarction based on bioinformatics
本研究通过生物信息学分析，探讨了心肌梗死（MI）中免疫和凝血相关基因的生物标志物。识别出IGF2R、TGFA、PLAUR和MAPK14作为关键生物标志物，强调其在MI病理生理中的重要性。研究结果为MI的早期诊断和个性化治疗提供了新的潜在靶点，具有重要的临床应用价值。
EVI5 unveils a role of the oncogene in modulating the Rab11/PD-L1 pathway in lung adenocarcinoma
本研究揭示了EVI5在肺腺癌中的新角色，表明其通过调节Rab11影响PD-L1的表达，从而可能在肿瘤免疫微环境中发挥作用。这一发现为克服免疫治疗抵抗提供了新的治疗靶点，强调了EVI5在肺腺癌治疗中的潜在重要性。
Circ_0003266 suppresses bladder cancer progression by modulating the miR-503-5p/SMAD7/TGF-β signaling axis
Circ_0003266 has been identified as a tumor suppressor in bladder cancer, with its expression inversely correlated with tumor size and disease stage. The study demonstrates that circ_0003266 inhibits cancer progression by regulating the miR-503-5p/SMAD7/TGF-β signaling pathway, highlighting its potential as a therapeutic target. In vivo experiments further confirm that circ_0003266 overexpression significantly reduces tumor growth, suggesting its relevance in bladder cancer treatment strategies.